Mesenchymal stem cells (MSC) is an important type of cell that is highly acclaimed for their safety and efficacy as a therapeutic agent cells. In order to obtain MSC, primary tissue (adipose tissue, bone marrow and cord blood) should be used; However, this network, especially the umbilical cord blood, it is difficult to obtain due to various reasons, such as low birth rate trend. In addition, to maximize the safety and efficacy of the MSC as an allogeneic cell therapy agent, it is desirable to minimize the possibility of immune rejection after transplantation in vivo.

This study attempts to establish a new method for producing induced pluripotent stem cells (IPSC) -derived MSC in which the human leukocyte antigen (HLA) -class I gene knocked out. To do this, derived dermal fibroblasts generation using Yamanaka 4 IPSC-factor, the HLA class I edited IPSC generation using CRISPR / Cas9, and differentiation of the IPSC to the MSC using the MSC culture medium utilized.

Through this, the HLA-A, B, and C pseudo-homozygous IPSC-derived MSCs (KO IMSC) produced by monoallelically knocked out of polymorphic HLA-A, B, and C genes, which are the main cause immune rejection for allogenic cell transplantation , Produced KO Imsc have multipotency and it is safe in vivo can be differentiated into cartilage.

Moreover, not attacked by natural killer cells do not like zero cells of HLA class I. In conclusion, KO Imsc which do not induce immune rejection during transplantation of allogeneic cells can be produced. In the future, KO Imsc can be successfully utilized as a therapeutic agent for multiple recipients of allogeneic cells via HLA typing.

Human Leukocyte Antigen Class I Pseudo-Homozygous Mesenchymal Stem Cells Derived from Human Induced Pluripotent Stem Cells
Human Leukocyte Antigen Class I Pseudo-Homozygous Mesenchymal Stem Cells Derived from Human Induced Pluripotent Stem Cells

human leukocyte antigen matching related donor for patients with sickle cell disease: results of international donors

Sickle cell disease (SCD) is the most common inherited hemoglobinopathies. hematopoietic stem cell transplantation (HCT) is the only curative therapy for SCD, but some patients will have a matched donor siblings. Patients with SCD mostly of African origin, and therefore tend to find a suitable unrelated donors in international registries. Using HaploStats, we estimate HLA haplotypes for 185 patients with SCD (n = 116 from the center of Brazil; n = 69 of the European Society for Blood and Marrow Transplantation (EBMT) centers) and haplotypes classified ethnic origin.

Next, we assess the probability of finding the human leukocyte antigen (HLA) matched unrelated adult donor (MUD), given the locus of A, B and DRB1 (6/6), in the international registries. Most haplotype is Africa, but Brazil showed the ethnic mix of higher than patients EBMT. Nevertheless, the possibility of finding at least one potential donor 6/6 allele was 47% for both groups. Most potential donors alleles derived from the National Marrow Donor Program (NMDP) Registry (USA) and from donor registry Brazil (REDOME).

Although the probability of finding a donor is higher than previously reported, the strategies needed to increase ethnic diversity in applicants. Furthermore, predicting the possibility of having MUD may influence the management of SCD.

Loss of human leukocyte antigen (HLA) class 1 expression is an escape mechanism of tumor immunity and may contribute to resistance to immunotherapy. Patients with lung cancer non-small cell (NSCLC) treated with immune checkpoint inhibitor may have discordant responses between brain metastases and extracranial sites of disease.

Anti-HLA-DR APC

1A-474-T100 100 tests
EUR 240

Anti-HLA-DR APC

1A-690-T025 25 tests
EUR 140

Anti-HLA-DR APC

1A-690-T100 100 tests
EUR 240

Anti-HLA-DR/DP APC

1A-296-T025 25 tests
EUR 140

Anti-HLA-DR/DP APC

1A-296-T100 100 tests
EUR 240

Anti-HLA-DR APC-Cy7

T4-690-T025 25 tests
EUR 154

Anti-HLA-DR APC-Cy7

T4-690-T100 100 tests
EUR 268

HLA-DR+DP Antibody (APC)

abx140299-100tests 100 tests
EUR 481

Mouse Anti Human Hla Dr Monoclonal Antibody,APC

DMABT-48803MH 100 TEST
EUR 767

anti-HLA-DR

YF-PA12351 50 ug
EUR 363
Description: Mouse polyclonal to HLA-DR

anti-HLA-DR

YF-PA12352 50 ul
EUR 363
Description: Mouse polyclonal to HLA-DR

anti-HLA-DR

YF-PA12353 50 ug
EUR 363
Description: Mouse polyclonal to HLA-DR

anti-HLA-DR

YF-PA12354 100 ul
EUR 403
Description: Rabbit polyclonal to HLA-DR

anti-HLA-DR

YF-PA12355 100 ug
EUR 403
Description: Rabbit polyclonal to HLA-DR

anti-HLA-DR

520-A-01mg 0,1 mg
EUR 267.5
Description: anti-HLA-DR

anti-HLA-DR

520-A-1000ug 1000 ug
EUR 1282.5
Description: anti-HLA-DR

HLA-DR Antigens-Associated Invariant Chain (HLA-DR) Antibody (APC)

abx200527-100tests 100 tests
EUR 634

HLA-DR Antigens-Associated Invariant Chain (HLA-DR) Antibody (APC)

abx140295-100tests 100 tests
EUR 481

HLA-DR Antigens-Associated Invariant Chain (HLA-DR) Antibody (APC)

abx140306-100tests 100 tests
EUR 481

Anti-HLA/DR antibody

STJ16100046 100 µg
EUR 406

Anti-HLA-DR/HLA-DRA Antibody

A01195 100ug/vial
EUR 334

HLA-DR Antigens-Associated Invariant Chain (HLA-DR) Antibody (APC-C750)

abx200532-100tests 100 tests
EUR 996

Anti-HLA-DR Antibody [L243], APC-Cy7-100Tests

QAB86-APC7-100Tests 100Tests
EUR 403

Anti-HLA-DR Antibody [L243], APC-Cy7-25Tests

QAB86-APC7-25Tests 25Tests
EUR 225

Anti-HLA-DR Purified

11-241-C025 0.025 mg
EUR 104

Anti-HLA-DR Purified

11-241-C100 0.1 mg
EUR 167

Anti-HLA-DR Purified

11-474-C025 0.025 mg
EUR 104

Anti-HLA-DR Purified

11-474-C100 0.1 mg
EUR 167

Anti-HLA-DR Purified

11-690-C025 0.025 mg
EUR 104

Anti-HLA-DR Purified

11-690-C100 0.1 mg
EUR 167

Anti-HLA-DR FITC

1F-474-T025 25 tests
EUR 122

Anti-HLA-DR FITC

1F-474-T100 100 tests
EUR 204

Anti-HLA-DR FITC

1F-690-T025 25 tests
EUR 122

Anti-HLA-DR FITC

1F-690-T100 100 tests
EUR 204

Anti-HLA-DR PE

1P-474-T025 25 tests
EUR 140

Anti-HLA-DR PE

1P-474-T100 100 tests
EUR 240

Anti-HLA-DR PE

1P-690-T025 25 tests
EUR 140

Anti-HLA-DR PE

1P-690-T100 100 tests
EUR 240

Anti-HLA-DR PerCP

PC-474-T025 25 tests
EUR 140

Anti-HLA-DR PerCP

PC-474-T100 100 tests
EUR 240

Anti-HLA-DR+DP antibody

STJ16100003 100 µg
EUR 406

Anti-HLA-DR antigen antibody

STJ16100045 1 mL
EUR 478

Anti-HLA-DR+DP antibody

STJ16100047 100 µg
EUR 406

Anti-HLA-DR+DP antibody

STJ16100576 100 tests
EUR 524

HLA-DR

HLADRA-100T 100 test
EUR 411.4

HLA-DR

HLADRAC750-100T 100 test
EUR 583

HLA-DR

HLADRB-01MG 100 test
EUR 284

HLA-DR

HLADRCFB-100T 100 test
EUR 388

HLA-DR

HLADRCFB2-100T 100 test
EUR 388

HLA-DR

HLADRF-100T 100 test
EUR 297

HLA-DR

HLADRPE-100T 100 test
EUR 349

HLA-DR

HLADRPP-100T 100 test
EUR 349

HLA-DR

HLADRPP5.5-100T 100 test
EUR 388

HLA-DR

HLADRPP5.52-100T 100 test
EUR 531

HLA-DR

HLADRPU-01MG 0,1 mg
EUR 219

HLA-DR antibody

23013-100ul 100ul
EUR 390

HLA-DR Antibody

48982-100ul 100ul
EUR 333

HLA-DR Antibody

48982-50ul 50ul
EUR 239

Anti-Human HLA-ABC Monoclonal Antibody APC Conjugated, Flow Validated

FC20056-APC 25 Tests, 100 Tests, 200 Tests
EUR 176
Description: Mouse Monoclonal Human HLA-ABC Antibody APC Conjugated, Flow Validated. Validated in Flow Cytometry and tested in Human.

Anti-HLA-DRA/Hla Dr Rabbit Monoclonal Antibody

M01195-2 100ug/vial
EUR 397
Description: Rabbit Monoclonal HLA-DRA/Hla Dr Antibody. Validated in IF, WB and tested in Human, Mouse, Rat.

Mouse Anti Human Hla Dr Monoclonal Antibody

DMABT-48801MH 0.2 mg
EUR 881

Mouse Anti Human Hla Dr Monoclonal Antibody

DMABT-48806MH 0.2 mg
EUR 767

Rat Anti Human Hla Dr Monoclonal Antibody

DMABT-48809RH 0.25 mg
EUR 767

Rat Anti Human Hla Dr Monoclonal Antibody

DMABT-48811RH 5 ml
EUR 580

HLA-DR Antigens-Associated Invariant Chain (HLA-DR) Antibody

abx413474-02mg 0.2 mg
EUR 565

HLA-DR Antigens-Associated Invariant Chain (HLA-DR) Antibody

abx415437-025mg 0.25 mg
EUR 565

HLA-DR Antigens-Associated Invariant Chain (HLA-DR) Antibody

abx415449-02mg 0.2 mg
EUR 565

HLA-DR Antigens-Associated Invariant Chain (HLA-DR) Antibody

abx200524-100ug 100 ug
EUR 328

HLA-DR Antigens-Associated Invariant Chain (HLA-DR) Antibody

abx140253-01mg 0.1 mg
EUR 370

HLA-DR Antigens-Associated Invariant Chain (HLA-DR) Antibody

abx140231-01mg 0.1 mg
EUR 370

HLA-DR Antigens-Associated Invariant Chain (HLA-DR) Antibody

abx140254-01mg 0.1 mg
EUR 370

HLA-DR Antigens-Associated Invariant Chain (HLA-DR) Antibody

abx140257-01mg 0.1 mg
EUR 370

Anti-HLA-DR/DP Purified

11-239-C025 0.025 mg
EUR 99

Anti-HLA-DR/DP Purified

11-239-C100 0.1 mg
EUR 158

Anti-HLA-DR/DP Purified

11-240-C025 0.025 mg
EUR 99

Anti-HLA-DR/DP Purified

11-240-C100 0.1 mg
EUR 158

Anti-HLA-DR/DP Purified

11-296-C025 0.025 mg
EUR 99

Anti-HLA-DR/DP Purified

11-296-C100 0.1 mg
EUR 158

Anti-HLA-DR/DP Biotin

1B-296-C025 0.025 mg
EUR 122

Anti-HLA-DR/DP Biotin

1B-296-C100 0.1 mg
EUR 204

Anti-HLA-DR/DP FITC

1F-239-T025 25 tests
EUR 122

Anti-HLA-DR/DP FITC

1F-239-T100 100 tests
EUR 204

Anti-HLA-DR/DP FITC

1F-296-T025 25 tests
EUR 122

Anti-HLA-DR/DP FITC

1F-296-T100 100 tests
EUR 204

Anti-HLA-DR/DP PE

1P-239-T025 25 tests
EUR 140

Anti-HLA-DR/DP PE

1P-239-T100 100 tests
EUR 240

Anti-HLA-DR/DP PE

1P-296-T025 25 tests
EUR 140

Anti-HLA-DR/DP PE

1P-296-T100 100 tests
EUR 240

Anti-HLA-DR Alexa Fluor647

A6-690-T025 25 tests
EUR 154

Anti-HLA-DR Alexa Fluor647

A6-690-T100 100 tests
EUR 269

Anti-HLA-DR Alexa Fluor700

A7-474-T025 25 tests
EUR 154

Anti-HLA-DR Alexa Fluor700

A7-474-T100 100 tests
EUR 269

Anti-HLA-DR PE-DyLight594

T5-690-T025 25 tests
EUR 154

Anti-HLA-DR PE-DyLight594

T5-690-T100 100 tests
EUR 268

Anti-HLA-DR PE-Cy7

T7-690-T025 25 tests
EUR 154

Anti-HLA-DR PE-Cy7

T7-690-T100 100 tests
EUR 268

Anti-HLA-DR PE-Cy5

T8-690-T025 25 tests
EUR 140

Anti-HLA-DR PE-Cy5

T8-690-T100 100 tests
EUR 240

Anti-HLA-DR PerCP-Cy5.5

T9-690-T025 25 tests
EUR 196

Anti-HLA-DR PerCP-Cy5.5

T9-690-T100 100 tests
EUR 352

Anti-HLA-DR Pacific Blue

PB-474-T025 25 tests
EUR 154

Anti-HLA-DR Pacific Blue

PB-474-T100 100 tests
EUR 269

Anti-HLA-DR Pacific Blue

PB-690-T025 25 tests
EUR 154

Anti-HLA-DR Pacific Blue

PB-690-T100 100 tests
EUR 269

Anti-HLA-DR Pacific Orange

PO-690-T025 25 tests
EUR 154

Anti-HLA-DR Pacific Orange

PO-690-T100 100 tests
EUR 269

HLA-DR Antibody (FITC)

abx200525-100tests 100 tests
EUR 398

HLA-DR Antibody (Biotin)

abx200529-100ug 100 ug
EUR 384

HLA-DR Antibody (FITC)

abx134336-100Assays 100 Assays
EUR 439

HLA-DR Antibody (FITC)

abx134336-50Assays 50 Assays
EUR 342

HLA-DR+DP Antibody

abx140228-01mg 0.1 mg
EUR 356

HLA-DR+DP Antibody

abx140232-01mg 0.1 mg
EUR 356

HLA-DR+DP Antibody

abx140233-01mg 0.1 mg
EUR 356

HLA-DR Antibody (FITC)

abx140296-100tests 100 tests
EUR 425

HLA-DR Antibody (FITC)

abx140304-100tests 100 tests
EUR 425

HLA DR beta antibody

23015-100ul 100ul
EUR 390

HLA-DR Conjugated Antibody

C48982 100ul
EUR 397

HLA-DR/CD34

HLADRF34PE-50T 50 test
EUR 323

HLA-DR/CD3

HLADRF3PE1-50T 50 test
EUR 350.3

HLA-DR/CD8

HLADRF8PE1-50T 50 test
EUR 350.3

HLA-DR Antigens-Associated Invariant Chain (HLA-DR) Antibody (RPE)

abx413475-100tests 100 tests
EUR 592

HLA-DR Antigens-Associated Invariant Chain (HLA-DR) Antibody (FITC)

abx415435-01mg 0.1 mg
EUR 509

HLA-DR Antigens-Associated Invariant Chain (HLA-DR) Antibody (RPE)

abx415436-100tests 100 tests
EUR 592

HLA-DR Antigens-Associated Invariant Chain (HLA-DR) Antibody (PE)

abx200526-100tests 100 tests
EUR 453

HLA-DR Antigens-Associated Invariant Chain (HLA-DR) Antibody (PerCP)

abx200528-100tests 100 tests
EUR 453

HLA-DR Antigens-Associated Invariant Chain (HLA-DR) Antibody (PerCP)

abx140293-100tests 100 tests
EUR 481

HLA-DR Antigens-Associated Invariant Chain (HLA-DR) Antibody (PE)

abx140305-100tests 100 tests
EUR 481

HLA-DR Antigens-Associated Invariant Chain (HLA-DR) Antibody (PE)

abx140294-100tests 100 tests
EUR 481

Mouse Anti Human Hla Dr Monoclonal Antibody,RPE

DMABT-48807MH 100 TEST
EUR 715

Rat Anti Human Hla Dr Monoclonal Antibody,FITC

DMABT-48808RH 0.1 mg
EUR 663

Rat Anti Human Hla Dr Monoclonal Antibody,RPE

DMABT-48810RH 100 TEST
EUR 710

Anti-HLA-DR Antibody [L243], PerCP-100Tests

QAB86-PCP-100Tests 100Tests
EUR 428

Anti-HLA-DR Antibody [L243], PerCP-25Tests

QAB86-PCP-25Tests 25Tests
EUR 225

Anti-HLA-DR Antibody [L243], PE-100Tests

QAB86-PE-100Tests 100Tests
EUR 251

Anti-HLA-DR Antibody [L243], PE-25Tests

QAB86-PE-25Tests 25Tests
EUR 141

Anti-HLA-DR Antibody [L243], PE-500Tests

QAB86-PE-500Tests 500Tests
EUR 893

Anti-HLA-DR Purified Low Endotoxin

12-690-C100 0.1 mg
EUR 167

Anti-HLA-DR/DP Alexa Fluor700

A7-296-T025 25 tests
EUR 154

Anti-HLA-DR/DP Alexa Fluor700

A7-296-T100 100 tests
EUR 269

Anti-HLA-DR/DP Pacific Blue

PB-296-T025 25 tests
EUR 154

Anti-HLA-DR/DP Pacific Blue

PB-296-T100 100 tests
EUR 269

HLA-DR Antigens-Associated Invariant Chain (HLA-DR) Antibody (CF-Blue)

abx200530-100tests 100 tests
EUR 481

HLA-DR Antigens-Associated Invariant Chain (HLA-DR) Antibody (OC-515)

abx200531-100tests 100 tests
EUR 467

HLA-DR Antigens-Associated Invariant Chain (HLA-DR) Antibody (PerCP-Cy5.5)

abx200533-100tests 100 tests
EUR 481

HLA-DR Antigens-Associated Invariant Chain (HLA-DR) Antibody (CF-Blue)

abx200534-100tests 100 tests
EUR 481

HLA-DR Antigens-Associated Invariant Chain (HLA-DR) Antibody (PerCP-Cy5.5)

abx200535-100tests 100 tests
EUR 773

HLA Class II Histocompatibility Antigen, DR Alpha Chain (HLA-DR) Antibody

20-abx134338
  • EUR 356.00
  • EUR 565.00
  • EUR 300.00
  • 100 ul
  • 200 ul
  • 50 ul

HLA-DR+DP Antibody (PE)

abx140298-100tests 100 tests
EUR 481

HLA-DR+DP Antibody (Biotin)

abx140300-01mg 0.1 mg
EUR 425

HLA-DR+DP Antibody (FITC)

abx140301-100tests 100 tests
EUR 425

HLA-DR+DP Antibody (FITC)

abx140302-100tests 100 tests
EUR 425

HLA-DR+DP Antibody (PE)

abx140297-100tests 100 tests
EUR 481

Anti-human HLA-DR Monoclonal Antibody Unconjugated, Flow Validated

FC00568 100µg, 500µg
EUR 114
Description: Mouse Monoclonal human HLA-DR Antibody Unconjugated, Flow Validated. Validated in Flow Cytometry and tested in Human.

Mouse Anti Human Hla Dp Dq Dr Monoclonal Antibody

DMABT-48793MH 0.2 mg
EUR 741

Mouse Anti Human Hla Dp Dq Dr Monoclonal Antibody

DMABT-48795MH 0.2 mg
EUR 767

Mouse Anti Human Hla Dr Monoclonal Antibody,Azide Free

DMABT-48804MH 1 mg
EUR 1261

HLA Class II Histocompatibility Antigen, DR Alpha Chain (HLA-DR) Antibody (PE)

abx134337-100Assays 100 Assays
EUR 495

HLA Class II Histocompatibility Antigen, DR Alpha Chain (HLA-DR) Antibody (PE)

abx134337-50Assays 50 Assays
EUR 398

HLA-DR Antigens-Associated Invariant Chain (HLA-DR) Antibody (PE-DyLight 594)

abx140339-100tests 100 tests
EUR 523

Anti-HLA-DR Antibody [L243], Qfluor 710-100Tests

QAB86-QF710-100Tests 100Tests
EUR 352

Anti-HLA-DR Antibody [L243], Qfluor 710-25Tests

QAB86-QF710-25Tests 25Tests
EUR 200

Anti-HLA-DP/-DR (MHC II) Monoclonal Antibody

M00487 100ug/vial
EUR 397
Description: Anti-HLA-DP/-DR (MHC II) Antibody

Anti-HLA-DP/ HLA-DR Antibody Clone Bra-14, Unconjugated-100ug

3115-MSM2-P1 100ug
EUR 428

Anti-HLA-G APC

1A-292-C025 0.025 mg
EUR 186

Anti-HLA-G APC

1A-292-C100 0.1 mg
EUR 373

HLA-DR / CD3 Antibody (FITC, PE)

abx200620-50tests 50 tests
EUR 425

HLA-DR / CD8 Antibody (FITC, PE)

abx200621-50tests 50 tests
EUR 425

HLA-DR / CD34 Antibody (FITC, PE)

abx200622-50tests 50 tests
EUR 425

Anti-human HLA-DR Monoclonal Antibody Biotin Conjugated, Flow Validated

FC00568-Biotin 100µg
EUR 233
Description: Mouse Monoclonal human HLA-DR Antibody Biotin Conjugated, Flow Validated. Validated in Flow Cytometry and tested in Human.

Anti-human HLA-DR Monoclonal Antibody FITC Conjugated, Flow Validated

FC00568-FITC 25 Tests, 100 Tests, 200 Tests
EUR 137
Description: Mouse Monoclonal human HLA-DR Antibody FITC Conjugated, Flow Validated. Validated in Flow Cytometry and tested in Human.

Anti-human HLA-DR Monoclonal Antibody PE Conjugated, Flow Validated

FC00568-PE 25 Tests, 100 Tests, 200 Tests
EUR 153
Description: Mouse Monoclonal human HLA-DR Antibody PE Conjugated, Flow Validated. Validated in Flow Cytometry and tested in Human.

Anti-human HLA-DR Monoclonal Antibody PerCP Conjugated, Flow Validated

FC00568-PerCP 100 Test, 200 Tests
EUR 342
Description: Mouse Monoclonal human HLA-DR Antibody PerCP Conjugated, Flow Validated. Validated in Flow Cytometry and tested in Human.

Mouse Anti Human Hla Dp Dq Dr Monoclonal Antibody,FITC

DMABT-48792MH 0.1 mg
EUR 663

Mouse Anti Human Hla Dp Dq Dr Monoclonal Antibody,FITC

DMABT-48794MH 0.1 mg
EUR 663

Mouse Anti Human Hla Dp Dq Dr Monoclonal Antibody,RPE

DMABT-48796MH 100 TEST
EUR 715

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 349

Rabbit Anti-HLA-DR monoclonal antibody, clone TD17-89

CABT-L707 100 ul
EUR 777

Anti-HLA-DP/-DQ/-DR (MHC II) Monoclonal Antibody

M30948 100ug/vial
EUR 397
Description: Mouse Monoclonal HLA-DP/-DQ/-DR (MHC II) Antibody. Validated in IHC and tested in Human.

Leukocyte Antigen DP/DQ/DR (HLA-DP/DQ/DR) Antibody

abx414274-02mg 0.2 mg
EUR 565

Leukocyte Antigen DP/DQ/DR (HLA-DP/DQ/DR) Antibody

abx414926-02mg 0.2 mg
EUR 565

ELISA kit for Rat Leukocyte antigen-DR (HLA-DR)

KTE100978-48T 48T
EUR 332
Description: Quantitative sandwich ELISA for measuring Rat Leukocyte antigen-DR (HLA-DR) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

We sought to evaluate whether the expression of HLA class 1 is maintained in metastatic NSCLC. NSCLC patients with paired primary and metastatic brain networks identified from the registry of our institution. HLA class 1 expression on the cell membrane of tumor cells is determined by immunohistochemistry.