Bioassay Products by Category
Real-Time PCR Kits
SensiMix Real-Time PCR Kits
A comprehensive range of highly optimized products: designed to deliver outstanding results
Validated with all major real-time instruments: generates reliable and highly reproducible data on all commonly used real-time PCR instruments
Powered by SensiTaq: a proprietary hot-start PCR enzyme that eliminates primer-dimer formation and non-specific amplification
Extremely fast protocols: ideal for the new generation of fast-cyclers
Flexible: developed for a variety of DNA and RNA templates including cDNA, poly(A) RNA, total RNA and genomic templates
Which kit do you need? Use the real-time PCR Reagent Selection Guide to select the most suitable product for your real-time PCR instrument.
Nucleic Acid Isolation
Designed for fast and efficient isolation of DNA and RNA from a wide range of biological materials, including animal tissue, cultured cells, buccal swabs, fecal material, bacterial cells, plant tissue, PCR products and agarose gels. ISOLATE Kits are based on a proprietary filter membrane spin column technology and follow a simple bind-wash-elute protocol. The isolated products are ready to use in downstream applications such as PCR, real-time PCR, cloning, sequencing, genotyping, etc. Use the Nucleic Acid Isolation Selection Table to choose the suitable ISOLATE kit for your samples.
New Bioline Crystal buffers for DNA/RNA and
protein electrophoresis are made from reagents
with the highest purity and reliability, Crystal
Buffers include 10x TBE, 50x TAE, 1x TG and 10x
TE in pouches and SDS Reagent and PBS Buffer in
Simply dissolve the contents of each pouch or place the tablet in deionized water and you are ready to go without any further preparation.
Pure. Simple. Convenient.
Bioline offers a range of high quality reagents for RNA analysis. The RNA Guide is designed to help you choose the right product for your RNA work. Along with detailed product descriptions, the guide also provides protocols, images, practical tips and troubleshooting ideas, making it a handy reference booklet for use in the laboratory.
VELOCITY DNA Polymerase
High-speed, high fidelity DNA polymerase
Shorter PCR runs for longer templates
Robust, requires minimal optimization of the reaction
Cloning techniques where high fidelity is desirable
Amplification of difficult templates
VELOCITY is a fast thermostable enzyme possessing 5΄-3΄ DNA polymerase and 3΄-5΄ proofreading exonuclease activities. VELOCITY provides high fidelity with an error-rate of 8 x 10-7 combined with a high speed DNA synthesis as fast as 15s/Kb for templates of up to 5Kb and 30s/Kb for templates longer than 5Kb. The inherent high speed DNA synthesis is due to the enhanced processivity of the polymerase
VELOCITY is easy to use since it works with many different protocols and requires minimal optimization. The polymerase produces higher yields than most commercially available enzymes and generates blunt-ended amplicons. The error rate of VELOCITY DNA Polymerase (8 x 10-7) was determined using the rpsL fidelity assay (Lackovich et al., 2001; Fujii et al., 1999).
One unit will incorporate 10nmoles of dNTPs in 30min at 72°C.
VELOCITY DNA Polymerase can be stored for 12 months at 20°C